GhrelinO-acyltransferase (GOAT), a specific enzyme that modifies ghrelin with a medium-chain fatty acid
نویسندگان
چکیده
منابع مشابه
A small molecule antagonist of ghrelin O-acyltransferase (GOAT).
Using our recently disclosed fluorescence-based assay to monitor acyltransferase activity, the first non-peptidic, small molecule antagonists of ghrelin O-acyltransferase (GOAT), a potential anti-obesity and anti-diabetes target, have been discovered. Each exhibits micromolar inhibition of the enzyme, and may be useful probes for future study of the ghrelin-GOAT system.
متن کاملMedium-chain fatty acid synthesis.
Most plant and mammalian tissue lipids contain fatty acids with a carbon chain longer than 14 atoms. However, milk lipids from several species like ruminants and rodents (Jenness, 1974), seed lipids from certain plants like coconuts (Child, 1974) and Cuphea (Graham & Kleiman, 1985) and lipids from several insects (AddaeMensah & Cameron, 1978) and from some bird glands (Downing, 1976; Jacob, 197...
متن کاملMedium chain fatty acids as specific substrates for diglyceride acyltransferase in cultured hepatocytes.
The fatty acyl substrate specificity for de novo diglyceride, triglyceride, and phospholipid synthesis in cultured hepatocytes was evaluated. The fatty acyl availability for diglyceride and phospholipid synthesis is restricted to fatty acids longer than myristic acid with octanoic and decanoic acids being excluded. On the other hand, octanoic and decanoic acids may serve as substrates for the d...
متن کاملInhibition of ghrelin O-acyltransferase (GOAT) by octanoylated pentapeptides.
The discovery of ghrelin O-acyltransferase (GOAT) opens the way to the design of drugs that block the attachment of an octanoyl group to the appetite-stimulating peptide hormone ghrelin, potentially preventing obesity. Here, we develop a biochemical assay that uses membranes from insect cells infected with baculovirus encoding mouse GOAT. The GOAT-containing membranes transferred the [(3)H]octa...
متن کاملCharacterization of Ghrelin O-Acyltransferase (GOAT) in goldfish (Carassius auratus)
Ghrelin is the only known hormone posttranslationally modified with an acylation. This modification is crucial for most of ghrelin's physiological effects and is catalyzed by the polytopic enzyme ghrelin O-acyltransferase (GOAT). The aim of this study was to characterize GOAT in a teleost model, goldfish (Carassius auratus). First, the full-length cDNA sequence was obtained by RT-PCR and rapid ...
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ژورنال
عنوان ژورنال: Journal of Biochemistry
سال: 2016
ISSN: 0021-924X,1756-2651
DOI: 10.1093/jb/mvw046